PHOSPHATE METABOLISM IN ERYTHROCYTES OF NORMAL HUMANS AND OF PATIENTS WITH HEREDITARY SPHEROCYTOSIS
Abstract
Erythrocytes of normal adults and of patients with hereditary spherocytosis were studied after in vitro incubation with P 32 orthophosphate. After 2 minutes' incubation much radioactivity was present in adenosine triphosphate and inorganic phosphate in contrast to a smaller amount in 2,3-diphosphoglyceric acid. Subsequent incubation with non-radioactive phosphate permitted observation of the fate of this radioactivity which was found to decrease in adenosine triphosphate and inorganic phosphate and rise in the 2,3-diphosphoglyceric acid pool. These observations suggest that the phosphate of adenosine triphosphate and inorganic phosphate may bear a precursor relationship to the phosphate of 2,3-diphosphoglyceric acid. No difference was observed between the erythrocytes of normal adults and of patients with hereditary spherocytosis.
Related Papers
- → Variable clinical severity of hereditary spherocytosis: Relation to erythrocytic spectrin concentration, osmotic fragility, and autohemolysis(1990)151 cited
- → Advances in laboratory diagnosis of hereditary spherocytosis(2016)55 cited
- → Hereditary Spherocytosis: Observations on Hemolytic Mechanisms and Iron Metabolism(1960)38 cited
- → Hereditary Spherocytosis-Sicklemia in the Negro(1959)16 cited
- INDIRECT HYPERBILIRUBINEMIA OF THE CHILD AGE AS A CONSEQUENCE OF SPHEROCYTOSIS(2000)