High efficiency of oxidative phosphorylation in mitochondria of wheat
Abstract
Mitochondria of young seedlings of a wheat hybrid 31MS × 28 exhibited high efficiency of oxidative phosphorylation. The ADP:O ratios estimated polarographically approached or were equivalent to 6, 4, and 5 with α-ketoglutarate, succinate, and malate, respectively. The respiratory control values with these substrates were about 7.5, 4.5, and 2.9. When assayed manometricafly, the P:O ratios with α-ketoglutarate as substrate and 10 min of reaction time were between 5.4 and 5.8. ADP was utilized almost exclusively in oxidative phosphorylation; microbial contamination in phosphorylating reaction mixtures had no measurable effect on oxidative phosphorylation. A concentration of 1.7 × 10 −5 M 2,4-dinitrophenol had no appreciable effect on stimulation of respiration by ADP. ATPase activity was increased about 13% by dinitrophenol. It appears that high efficiency of oxidative phosphorylation is a true characteristic of the mitochondria used in this study.
Related Papers
- → The effect of 2,4-dinitrophenol on the electrical resistance of phospholipid bilayer membranes(1966)163 cited
- → The Mechanism of Uncoupling of Oxidative Phosphorylation by 2,4-Dinitrophenol(1967)41 cited
- The analysis of drug action on mitochondrial oxidative phosphorylation. The choice of organic solvent for water-insoluble drugs.(1983)
- → A ‘latent’ dinitrophenol-stimulated ATPase in red-cell ghosts(1967)12 cited
- → Bioenergetics and cell communication; cell uncoupling seen as a protective mechanism in cardiac muscle(1992)3 cited