Breast Cancer Cell Imaging using Semiconductor Quantum Dots
Citations Over TimeTop 22% of 2009 papers
Abstract
We report the development of early stage detection of breast cancer cells using quantum dots (QDs) as fluorescent signal generator for detection. The QDs based imaging of breast cancer cells involved two kinds of antibodies: the first was for labeling the cells and the second was for imaging the breast cancer cells. Anti-Her2/neu (10Ab) was used for labeling the captured SK-BR3 cells. The second antibody against anti-Her2/neu (20Ab) that was conjugated to QDs (20Ab~QDs) forms the complete assay, SK-BR3 + 10Ab + 20Ab~QDs, to generate the fluorescent cells for imaging. Fluorescent images of the complete assay for SK-BR3 cells were evaluated under a microscope with a UV light source. The preliminary results showed that the breast cancer cell SK-BR3 in the complete assay were successfully observed as fluorescent cells that had brighter signals compared with those labeled with organic dye using similar parameters and the same number of cells.
Related Papers
- → Fluorescence versus conventional sputum smear microscopy for tuberculosis: a systematic review(2006)787 cited
- → Fundamentals of Fluorescence Microscopy: Exploring Life with Light(2013)32 cited
- → Fluorescence Microscopy(2019)7 cited
- → The Dual-Mode Imaging of Nanogold-Labeled Cells by Photoacoustic Microscopy and Fluorescence Optical Microscopy(2018)3 cited
- [Comparative study of microscopy by the Ziehl Neelsen method, routine fluorescence microscopy, and fluorescence microscopy using a lumin attachment in the diagnosis of acid-resistant mycobacteria].(2009)