Supplemental N‐3 Polyunsaturated Fatty Acids Limit A1‐Specific Astrocyte Polarization via Attenuating Mitochondrial Dysfunction in Ischemic Stroke in Mice

Citations Over TimeTop 13% of 2021 papers

Abstract

Ischemic stroke is one of the leading causes of death and disability for adults, which lacks effective treatments. Dietary intake of n-3 polyunsaturated fatty acids (n-3 PUFAs) exerts beneficial effects on ischemic stroke by attenuating neuron death and inflammation induced by microglial activation. However, the impact and mechanism of n-3 PUFAs on astrocyte function during stroke have not yet been well investigated. Our current study found that dietary n-3 PUFAs decreased the infarction volume and improved the neurofunction in the mice model of transient middle cerebral artery occlusion (tMCAO). Notably, n-3 PUFAs reduced the stroke-induced A1 astrocyte polarization both in vivo and in vitro. We have demonstrated that exogenous n-3 PUFAs attenuated mitochondrial oxidative stress and increased the mitophagy of astrocytes in the condition of hypoxia. Furthermore, we provided evidence that treatment with the mitochondrial-derived antioxidant, mito-TEMPO, abrogated the n-3 PUFA-mediated regulation of A1 astrocyte polarization upon hypoxia treatment. Together, this study highlighted that n-3 PUFAs prevent mitochondrial dysfunction, thereby limiting A1-specific astrocyte polarization and subsequently improving the neurological outcomes of mice with ischemic stroke.

Related Papers

• → Inhibition of NADPH oxidase promotes alternative and anti‐inflammatory microglial activation during neuroinflammation(2011)218 cited
• → Astrocyte-mediated control of cerebral microcirculation(2003)182 cited
• → Class I phosphoinositide 3-kinases control sustained NADPH oxidase activation in adherent neutrophils(2020)18 cited
• → Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models(2012)15 cited
• Antiarrhythmic effects of n-3 polyunsaturated fatty acids.(2005)