Proteome Analysis of Lens Epithelia, Fibers, and the HLE B-3 Cell Line
Investigative Ophthalmology & Visual Science2003Vol. 44(11), pp. 4829–4829
Citations Over TimeTop 22% of 2003 papers
S.T. Wang–Su, Ashley L. McCormack, Shaojun Yang, Matthew R. Hosler, April E. Mixon, Michael A. Riviere, Phillip A. Wilmarth, Usha P. Andley, Donita Garland, Hong Li, Larry L. David, B.J. Wagner
Abstract
Freshly dissected noncultured HLE cells from both central and peripheral regions contain a high concentration of crystallins that mask the detection of less abundant proteins by 2-DE. Transformation and culture of HLE cells causes a loss of these crystallins and an increase in the relative concentration of other proteins. However, most of these noncrystallin proteins were different from those observed in noncultured HLE cells. These results suggest that transformation markedly alters the protein expression pattern in immortalized HLE cells and that caution should be exercised when using them to study properties of HLE cells in vivo.
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