TheRd8Mutation of theCrb1Gene Is Present in Vendor Lines of C57BL/6N Mice and Embryonic Stem Cells, and Confounds Ocular Induced Mutant Phenotypes
Investigative Ophthalmology & Visual Science2012Vol. 53(6), pp. 2921–2921
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Mary J. Mattapallil, Eric F. Wawrousek, Chi‐Chao Chan, Hui Zhao, Jayeeta Roychoudhury, Thomas A. Ferguson, Rachel R Caspi
Abstract
These findings identify the presence of the rd8 mutation in the C57BL/6N mouse substrain used widely to produce transgenic and knockout mice. The results have grave implications for the vision research community who develop mouse lines to study eye disease, as presence of rd8 can produce significant disease phenotypes unrelated to the gene or genes of interest. It is suggested that researchers screen for rd8 if their mouse lines were generated on the C57BL/6N background, bear resemblance to the rd8 phenotype, or are of indeterminate origin.
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