Development of microsatellite multiplexes for wild goats using primers designed from domestic Bovidae
Citations Over Time
Abstract
Many wild goat taxa (Capra spp.) are endangered and would benefit from the availability of molecular tools that are useful for population management and conservation. We developed microsatellite DNA markers useful in all wild goat species, by using a cross-species amplification approach. Seventy-five microsatellite primer pairs designed from domestic cattle (Bos taurus), sheep (Ovis aries) and goat (Capra hircus) were tested on three distantly related Capra species: C. ibex ibex, C. [i.] sibirica, and C. pyrenaica. On average, 90% of the domestic ungulate primers amplified a microsatellite PCR product in the wild goat species. Forty percent of the total were polymorphic in C. i. ibex, which is expected to have the lowest genetic diversity among all Capra species. We developed multiplexes of 24 polymorphic fluorescent microsatellite loci that can be amplified in 13 PCR reactions and loaded into two gel-lanes. These microsatellites will allow studies of conservation and population ecology in all Capra species, and the multiplexes will reduce the time and cost of the genetic analyses.
Related Papers
- → Seasonal Prevalence of Gastro-Intestinal Helminths of Sheep (Ovis Aries) and Goat (Capra Hircus) with Respect to Age and Gender of Gurez Valley, Kashmir(2016)5 cited
- [Comparative study of the dissemination of gastrointestinal nematodes in domestic ruminants (Ovis aries and Capra hircus) and wild ruminants (Capra ibex and Rupicapra rupicapra in the Western mountain regions].(1978)
- Histomorphological study on placentomes of goat (Capra hircus) and sheep (Ovis aries) during various phases of gestation(2007)
- Shape of cavitas glenoidalis as a criterion in the differentiation of capra and ovis by the remains of os scapula capra falconeri capra sibirica capra hircus ovis ammon bochariensis ovis ammon polii ovis aries(1968)
- → A method for the genetic discrimination between species of goat (Capra hircus) and sheep (Ovis aries)(2012)