Mass Spectrometry of Peptides

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Abstract

INTRODUCTION. PREFACE. SECTION I: IONIZATION METHODS. 252Cf PLASMA DESORPTION METHODS. Fundamental Aspects of Protein Mass Spectrometry Using 252-Californium Plasma Desorption. Plasma Desorption Mass Spectrometry of Peptides and Peptide Conjugates. The Analysis of Synthetic Peptides and Proteins. Analysis of Peptides and Proteins by Plasma Desorption Mass Spectrometry. LASER-INDUCED MULTIPHOTON IONIZATION. Laser-Induced Multiphoton Ionization of Peptides in Supersonic Beam/Mass Spectrometry. SECTION II: INSTRUMENTAL DEVELOPMENTS. MAGNETIC SECTOR INSTRUMENTS. The Molecular Weight Determination of Large Peptides by Magnetic Sector Mass Spectrometry. Four-Sector Tandem Mass Spectrometry of Peptides. QUADRUPOLE FOURIER TRANSFORM. Peptide Sequence Analysis by Triple Quadrupole and Quadrupole Fourier Transform Mass Spectrometry. TIME-OF-FLIGHT INSTRUMENTS. Correlation Measurements in a Reflecting Time-of-Flight Mass Spectrometer. SECTION III: ANALYSIS OF PEPTIDES. SAMPLE PREPARATION. Sample Preparation and Matrix Selection for Analysis of Peptides by FAB and Liquid SIMS. LC-MS ANALYSIS. On-Line Methods for Peptide Analysis by Continuous-Flow FABMS. ANALYSIS OF PROTEIN PRODUCTS. Investigation of Amino Acid Mutation by High Resolution Mass Spectrometry. The Mass Spectral Analysis of Hemoglobin Variants. PROTEIN CROSS-LINKAGES. Detection and Location of Disulfide Bonds in Proteins by Mass Spectrometry. PEPTIDE INTERACTIONS WITH METAL IONS. Tandem Mass Spectrometry for Determining the Amino Acid Sequence of Cyclic Peptides and for Assessing Interactions of Peptides and Metal Ions. PERMETHYLATED PEPTIDES. Analysis of Permethylated Peptides by Mass Spectrometry. NEUROPEPTIDES. Applications of Mass Spectrometry for Characterization of Neuropeptides. Peptide-Charting Applied to Studies of Precursor Processing in Endocrine Tissues. QUANTIFICATION OF NEUROPEPTIDES. Mass Spectrometry of Biologically Important Neuropeptides

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