Involvement of Oct3/4 in the enhancement of neuronal differentiation of ES cells in neurogenesis-inducing cultures
Citations Over TimeTop 10% of 2003 papers
Abstract
Oct3/4 plays a critical role in maintaining embryonic stem cell pluripotency. Regulatable transgene-mediated sustained Oct3/4 expression in ES cells cultured in serum-free LIF-deficient medium caused accelerated differentiation to neuroectoderm-like cells that expressed Sox2, Otx1 and Emx2 and subsequently differentiated into neurons. Neurogenesis of ES cells is promoted by SDIA (stromal cell-derived inducing activity), which accumulates on the PA6 stromal cell surface. Oct3/4 expression in ES cells was maintained by SDIA whereas without it expression was promptly downregulated. Suppression of Oct3/4 abolished neuronal differentiation even after stimulation by SDIA. In contrast, sustained upregulated Oct3/4 expression enhanced SDIA-mediated neurogenesis of ES cells. Therefore, Oct3/4 appears to promote neuroectoderm formation and subsequent neuronal differentiation from ES cells.
Related Papers
- → Genetic influences on exercise-induced adult hippocampal neurogenesis across 12 divergent mouse strains(2011)98 cited
- → Adult Hippocampal Neurogenesis as Target for the Treatment of Depression(2007)129 cited
- → Adult neurogenesis research in China(2023)5 cited
- Factors influencing adult neurogenesis(2006)
- [The function of fatty acid signals in neurogenesis].(2011)