Multidrug resistance-associated protein MRP-1 regulates dauer diapause by its export activity inCaenorhabditis elegans
Citations Over Time
Abstract
Multidrug resistance-associated proteins (MRPs), when overexpressed, confer drug resistance to cancer cells by exporting anti-cancer agents through the cell membrane, but their role in animal development has not been elucidated. Here we show that an MRP homolog regulates larval development in the nematode Caenorhabditis elegans. C. elegans forms a special third-stage larva called a dauer larva under conditions inappropriate for growth. By contrast, we found that mutants in mrp-1, an MRP homolog gene, form dauer larvae even under conditions appropriate for growth, in the background of certain mutations that partially block the insulin signaling pathway. A functional mrp-1::GFP gene was shown to be expressed in many tissues, and the wild-type mrp-1 gene must be expressed in multiple tissues for a wild-type phenotype. Human MRP1 could substitute for C. elegans MRP-1 in dauer larva regulation, and an inhibitor of the human MRP1 transport activity impaired this function, showing that export activity is required for normal dauer larva regulation. Epistasis studies revealed that MRP-1 acts in neither the TGF-beta nor the cGMP signaling pathway. mrp-1 mutations enhanced the dauer-constitutive phenotype of mutants in the insulin signaling pathway more strongly than that in other pathways. Thus, MRP-1, through its export activity, supports the induction of the normal (non-dauer) life cycle by the insulin signaling pathway.
Related Papers
- → Effects of temperature and photoperiod on termination of pseudopupal diapause in the bean blister beetle, Epicauta gorhami(2012)44 cited
- → Summer diapause intensity influenced by parental and offspring environmental conditions in the pest mite, Halotydeus destructor(2019)27 cited
- → On the factors inducing the inhibition of diapause in the progeny of diapause females of Trichogramma telengai(2017)23 cited
- → Variation in diapause potential and strength in eggs of the Australian plague locust, Chortoicetes terminifera (Walker) (Orthoptera: Acrididae)(1984)21 cited
- Diapause conditions and diapause-associated proteins in Mylabris cichorii Linnaeus eggs(2011)