Developmental fate and lineage commitment of singled mouse blastomeres

Citations Over TimeTop 10% of 2012 papers

Abstract

The inside-outside model has been invoked to explain cell-fate specification of the pre-implantation mammalian embryo. Here, we investigate whether cell-cell interaction can influence the fate specification of embryonic blastomeres by sequentially separating the blastomeres in two-cell stage mouse embryos and continuing separation after each cell division throughout pre-implantation development. This procedure eliminates information provided by cell-cell interaction and cell positioning. Gene expression profiles, polarity protein localization and functional tests of these separated blastomeres reveal that cell interactions, through cell position, influence the fate of the blastomere. Blastomeres, in the absence of cell contact and inner-outer positional information, have a unique pattern of gene expression that is characteristic of neither inner cell mass nor trophectoderm, but overall they have a tendency towards a 'trophectoderm-like' gene expression pattern and preferentially contribute to the trophectoderm lineage.

Related Papers

• → Relationship between timing of development, morula morphology, and cell allocation to inner cell mass and trophectoderm in in vitro-produced bovine embryos(1997)269 cited
• → Methods for Derivation of Human Embryonic Stem Cells(2005)84 cited
• → Relationship between timing of development, morula morphology, and cell allocation to inner cell mass and trophectoderm in in vitro‐produced bovine embryos(1997)14 cited
• → Total Cell Number and its Allocation to Trophectoderm and Inner Cell Mass in In Vitro Obtained Cats' Blastocysts(2016)8 cited
• → Morphology and/or hatching ability of in vitro produced bovine embryos is no reliable indicator of inner cell mass cell number(1997)5 cited