In situ analysis of fetal, prepuberal and adult XX ↔ XY chimaeric mouse testes: Sertoli cells are predominantly, but not exclusively, XY

Citations Over TimeTop 10% of 1991 papers

Abstract

The testes of fetal, prepuberal and adult XX----XY chimaeras were examined using in situ hybridisation to identify the beta-globin transgenic marker contained in one component of each chimaera. This enabled the proportion of XX and XY cells contributing to the major cell lineages of the testis to be estimated from sectioned and air-dried material. A few XX Sertoli cells were found in all three age groups, but the XX contribution was always much lower than in other somatic cell types. Significantly, in fetal XX----XY testes, Sertoli cells were the only cell type to show a bias in favour of the XY component. This strengthens the view that Tdy acts solely in the lineage that gives rise to Sertoli cells. However, the finding of some fetal XX Sertoli cells means that one of the steps in the Tdy-initiated process of Sertoli cell determination is capable of locally recruiting XX cells.

Related Papers

• → Three‐dimensional reconstruction of a rat stage V Sertoli cell: II. Morphometry of Sertoli–Sertoli and Sertoli–germ‐cell relationships(1983)172 cited
• → A Unique Cytoplasmic Marker for Extratesticular Sertoli Cells(2002)16 cited
• → A new approach to quantification of sertoli cells that avoids problems associated with the irregular nuclear surface(1986)25 cited
• Culture and Biological Studies on Sertoli Cells Isolated from Mouse Testis(2008)
• Primary Culture and Identification of Rat Testicular Sertoli Cells in Vitro and Expression of Inhibin Inside the Cells(2006)