GLP-1 is localized to the mitotic region of the C. elegans germ line
Citations Over TimeTop 10% of 1994 papers
Abstract
In C. elegans, germline mitosis depends on induction by the somatic distal tip cell (DTC) and on activity of the glp-1 gene. Using antibodies to GLP-1 protein, we have examined GLP-1 on western blots and by immunocytochemistry. GLP-1 is tightly associated with membranes of mitotic germline cells, supporting its identification as an integral membrane protein. Furthermore, GLP-1 is localized within the germ line to the mitotic region, consistent with the model that GLP-1 acts as a membrane receptor for the distal tip cell signal. Unexpectedly, GLP-1 and the zone of mitosis extend further than the DTC processes. We present three models by which the DTC may influence GLP-1 activity and thereby determine the zone of mitosis. The spatial restriction of GLP-1 appears to be controlled at the translational level in hermaphrodites. We suggest that down-regulation of GLP-1 may be required to effect the transition from mitosis into meiosis.
Related Papers
- → MEG-1 and MEG-2 Are Embryo-Specific P-Granule Components Required for Germline Development in Caenorhabditis elegans(2008)40 cited
- → Chapter 4 Drosophila Germline Sex Determination: Integration of Germline Autonomous Cues and Somatic Signals(2008)21 cited
- Study of the Role of EGL-38 PAX in the Developing Egg-Laying System and Germline Cell Survival in Caenorhabditis Elegans(2007)
- Identification of antioxidant gene in Caenorhabditis elegans through computational approaches(2010)
- → ПОСЛЕДСТВИЯ КРАТКОВРЕМЕННОГО ДЕЙСТВИЯ ИОНОВ Pb2+, Cd2+ И Cu2+ НА ОРГАНИЗМ СВОБОДНОЖИВУЩЕЙ ПОЧВЕННОЙ НЕМАТОДЫ CAENORHABDITIS ELEGANS(2022)