Two functionally redundant sources of retinoic acid secure spermatogonia differentiation in the seminiferous epithelium

Citations Over TimeTop 10% of 2018 papers

Abstract

In mammals, all-trans retinoic acid (ATRA) is instrumental to spermatogenesis. It is synthesized by two retinaldehyde dehydrogenases (RALDH) present in both Sertoli cells (SCs) and germ cells (GCs). In order to determine the relative contributions of each source of ATRA, we have generated mice lacking all RALDH activities in the seminiferous epithelium (SE). We show that both the SC- and GC-derived sources of ATRA cooperate to initiate and propagate spermatogenetic waves at puberty. In adults, they exert redundant functions and, against all expectations, the GC-derived source does not perform any specific roles despite contributing to two-thirds of the total amount of ATRA present in the testis. The production from SCs is sufficient to maintain the periodic expression of genes in SCs, as well and the cycle and wave of the SE, which account for the steady production of spermatozoa. The production from SCs is also specifically required for spermiation. Importantly, our study shows that spermatogonia differentiation depends upon the ATRA synthesized by RALDH inside the SE, whereas initiation of meiosis and expression of STRA8 by spermatocytes can occur without ATRA.

Related Papers

• → The central role of Sertoli cells in spermatogenesis(1998)863 cited
• → Ultrastructural Observations of Spermatogenesis Following Transplantation of Rat Testis Cells Into Mouse Seminiferous Tubules(1996)112 cited
• → Spermatogonial proliferation patterns in men with azoospermia of different etiologies(2003)36 cited
• Spermatogenesis in Omocestus viridulus(2005)
• [Role of Sertoli cells in regulating spermatogenesis: An update].(2018)