mRBPome capture identifies the RNA-binding protein TRIM71, an essential regulator of spermatogonial differentiation
Citations Over TimeTop 24% of 2020 papers
Abstract
Continual spermatogenesis relies on the actions of an undifferentiated spermatogonial population that is composed of stem cells and progenitors. Here, using mouse models, we explored the role of RNA-binding proteins (RBPs) in regulation of the biological activities of this population. Proteins bound to polyadenylated RNAs in primary cultures of undifferentiated spermatogonia were captured with oligo (dT)-conjugated beads after UV-crosslinking and profiled by proteomics (termed mRBPome capture), yielding a putative repertoire of 473 RBPs. From this database, the RBP TRIM71 was identified and found to be expressed by stem and progenitor spermatogonia in prepubertal and adult mouse testes. Tissue-specific deletion of TRIM71 in the male germline led to reduction of the undifferentiated spermatogonial population and a block in transition to the differentiating state. Collectively, these findings demonstrate a key role of the RBP system in regulation of the spermatogenic lineage and may provide clues about the influence of RBPs on the biology of progenitor cell populations in other lineages.
Related Papers
- → Specificity factors in cytoplasmic polyadenylation(2013)161 cited
- → PolyA_DB: a database for mammalian mRNA polyadenylation(2004)134 cited
- → Musashi 1 regulates the timing and extent of meiotic mRNA translational activation by promoting the use of specific CPEs(2017)42 cited
- → Identifying RBP Targets with RIP-seq(2015)12 cited
- → The landscape of alternative polyadenylation during EMT and its regulation by the RNA-binding protein Quaking(2022)1 cited