High-resolution dynamic mapping of the C. elegans intestinal brush border

Citations Over Time

Abstract

The intestinal brush border is made of an array of microvilli that increases the membrane surface area for nutrient processing, absorption and host defense. Studies on mammalian cultured epithelial cells have uncovered some of the molecular players and physical constraints required to establish this apical specialized membrane. However, the building and maintenance of a brush border in vivo has not yet been investigated in detail. Here, we combined super-resolution imaging, transmission electron microscopy and genome editing in the developing nematode Caenorhabditis elegans to build a high-resolution and dynamic localization map of known and new brush border markers. Notably, we show that microvilli components are dynamically enriched at the apical membrane during microvilli outgrowth and maturation, but become highly stable once microvilli are built. This new toolbox will be instrumental for understanding the molecular processes of microvilli growth and maintenance in vivo, as well as the effect of genetic perturbations, notably in the context of disorders affecting brush border integrity.

Related Papers

• → Device for the control and measurement of brush forces to an accuracy of a few grams-force while monitoring brush resistance and brush wear at currents up to 500 A or more(1981)11 cited
• → On Some Results of Impact Test on Carbon Brush for an Electric Car(1953)
• Design of Contact Pressure Measurement System for Brush Probe(2011)
• → J044033 Frictional analysis of hydrophilic PVA brush for cleaning(2013)
• → ИСПОЛЬЗОВAНИЕ ПОТЕНЦИAЛA СОЦИAЛЬНЫХ ПAРТНЕРОВ В ПОДГОТОВКЕ БУДУЩИХ ПЕДAГОГОВ(2024)