Age-associated Decrease of Oxidative Repair Enzymes, Human 8-Oxoguanine DNA Glycosylases (hOgg1), in Human Aging

Citations Over TimeTop 22% of 2003 papers

Abstract

8-Oxoguanine has been shown to be a dominant cause of oxidative DNA damage by oxygen free radicals in eukaryotic cells. The 8-oxoguanine repair-specific enzyme 8-oxoguanine-DNA glycosylase (hOgg1) was recently cloned and was observed to conduct mainly short-patch base-excision repair. It has also been suggested that reactive oxygen species play an important role in the cellular aging process. We explored the association between the hOgg1 enzyme activity in somatic cells of human subjects of various ages and the role of hOgg1(326) genetic polymorphism. An 8-oxoguanine-containing 28 mer oligonucleotide was end-labeled with gamma-32P ATP and incubated with protein extracts from peripheral blood lymphocytes (PBL) from 78 healthy individuals ranging in age from newborn to 91 years old. The hOgg1 repair activity toward the radiolabelled 8-oxoguanine-containing DNA was determined, and the results indicated a significant age-dependent decrease in the hOgg1 activity in their lymphocytes. Significantly reduced activity was also shown in those with Cysteine/Cysteine genotypes. The genders of the subjects were not shown to be associated. These results provide an important observation regarding the cellular hOgg1 activity in somatic cells during the normal human aging processes.

Related Papers

• → Adenine DNA glycosylase activity of 14 Human MutY homolog (MUTYH) variant proteins found in patients with colorectal polyposis and cancer(2010)42 cited
• → Oxidative Damage in Sporadic Colorectal Cancer: Molecular Mapping of Base Excision Repair Glycosylases MUTYH and hOGG1 in Colorectal Cancer Patients(2022)12 cited
• Functional cooperation of Ogg1 and Mutyh in preventing G: C-->T: a transversions in mice.(2004)
• → Role of base excision repair DNA glycosylases in hereditary and infectious human diseases(2008)11 cited
• [The role of glycosylases of the base excision DNA repair in pathogenesis of hereditary and infectious human diseases].(2009)