Anti-Aging Effects of Phloridzin, an Apple Polyphenol, on Yeastviathe SOD and Sir2 Genes

Citations Over TimeTop 13% of 2011 papers

Abstract

The anti-aging effects of phloridzin on the yeast Saccharomyces cerevisiae were investigated by employing a replicative lifespan assay of the K6001 yeast strain. After administrating phloridzin at doses of 3, 10, and 30 µM, the lifespan of the yeast was significantly prolonged in comparison with the untreated group (p<0.01, p<0.001). To determine the mechanism of action, anti-oxidative experiments and ROS assay were performed. Phloridzin improved the viability of the yeast dose-dependently under oxidative stress by 7.5 mM H(2)O(2), and a low dose of phloridzin inhibited ROS of the yeast. Further, SOD1, SOD2, and Sir2 gene expression was examined by reverse transcription-polymerase chain reaction (RT-PCR), and was found to be significantly increased. Finally, superoxide dismutase (SOD) and SIRT1 activity assays showed that phloridzin notably increased the activity of SOD and SIRT1. These results suggest that SOD and Sir2 have important roles in phloridzin-regulated lifespan extension of yeast, and potentially anti-aging effects for mammalian cells via SIRT1.

Related Papers

• → A correlation of reactive oxygen species accumulation by depletion of superoxide dismutases with age-dependent impairment in the nervous system and muscles of Drosophila adults(2015)75 cited
• → Mutant SOD1‐induced neuronal toxicity is mediated by increased mitochondrial superoxide levels(2007)60 cited
• → Development of novel small molecules for the treatment of ALS(2020)8 cited
• → Adaptation mechanism of yeast to extreme environments: Construction of salt-tolerance mutants of the yeast Saccharomyces cerevisiae(1992)15 cited
• → Physical and biochemical properties of freeze-tolerant mutants of a yeast Saccharomyces cerevisiae(1990)15 cited