Fungal Glucuronoyl Esterases and Substrate Uronic Acid Recognition
Citations Over TimeTop 14% of 2009 papers
Abstract
Glucuronoyl esterases are enzymes involved in microbial plant cell-wall degradation. In this study we purified and characterized two recombinant Phanerochaete chrysosporium glucuronoyl esterases, PcGE1 and PcGE2. The catalytic activity of these and previously described glucuronoyl esterases was investigated on new synthetic substrates, methyl esters of uronic acids and their glycosides, prepared by esterification with ethereal diazomethane. The data obtained indicate that the enzymes hydrolyzed efficiently not only esters of 4-O-methyl-d-glucuronic acid, but also methyl esters of d-glucuronic acid carrying a 4-nitrophenyl aglycon. Moreover, the fact that they did not recognize the 4-epimers of these compounds, the d-galacturonic acid derivatives, supports the hypothesis that these carbohydrate esterases attack ester linkages between 4-O-methyl-d-glucuronic acid of glucuronoxylan and lignin alcohols.
Related Papers
- → Biodegradation of sulfamethoxazole by Phanerochaete chrysosporium(2014)56 cited
- → Development of a bioreactor system using an immobilized white rot fungus for decolorization(1996)32 cited
- → [27] Lignin peroxidase from fungi: Phanerochaete chrysosporium(1990)54 cited
- → Influence of heavy-metals toxicity on the growth of Phanerochaete chrysosporium(1997)40 cited
- Application and a Study on the Degradation Mechanism of Phanerochaete Chrysosporium(2003)