A Defined and Xeno-Free Culture Method Enabling the Establishment of Clinical-Grade Human Embryonic, Induced Pluripotent and Adipose Stem Cells
PLoS ONE2010Vol. 5(4), pp. e10246–e10246
Citations Over TimeTop 10% of 2010 papers
Kristiina Rajala, Bettina Lindroos, Samer M. I. Hussein, Riikka S. Lappalainen, Mari Pekkanen-Mattila, José Inzunza, Björn Rozell, Susanna Miettinen, Susanna Narkilahti, Erja Kerkelä, Katriina Aalto‐Setälä, Timo Otonkoski, Riitta Suuronen, Outi Hovatta, Heli Skottman
Abstract
Our results demonstrate that human ESCs, iPSCs and ASCs can be maintained in the same defined xeno-free medium formulation for a prolonged period of time while maintaining their characteristics, demonstrating the applicability of the simplified xeno-free medium formulation for the production of clinical-grade stem cells. The basic xeno-free formulation described herein has the potential to be further optimized for specific applications relating to establishment, expansion and differentiation of various stem cell types.
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