A Study on Melatonin Receptor Genes Expression in Embryonic Goose Skin Feather Follicles
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Abstract
Background: In recent years, it has been reported that melatonin plays an important role in the development of skin hair follicles in mammals. The down we often use is mainly from the skin feather follicles of geese, but their production is not high. Therefore, the study of skin feather follicles is of great significance. Methods: The skin feather follicle tissues of goose dorsal skin at three developmental nodes (E14, E18 and E28) were collected; the differential expression and distribution of melatonin receptor genes were detected by fluorescence quantitative PCR, Western Blot and immunohistochemical methods, respectively. The results showed that melatonin receptor gene was expressed in varying degrees at three time points of goose embryonic skin feather follicle development. The expression sites and Protein expression level of Mel1a and RORα were regularly changed by immunohistochemistry and Western Blot. Result: Our research showed that melatonin receptor genes can play an important regulatory role in the development of goose skin feather follicles.
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