Development of GFAP positive Cells and Reactive Changes Associated with Cystic Lesions in HTX Rat Brain

Citations Over Time

Abstract

HTX rat, a congenital hydrocephalic strain, develops ventricular dilatation and cystic cavities in the cerebral white matter after birth. To investigate the reactive changes in glial cells around these cavities, immunohistochemical staining for glial fibrillary acidic protein (GFAP), a specific marker protein of astrocytes, and bromodeoxyuridine (BrdU), a thymidine analogue, was carried out on 107 Wistar and HTX rat brains from birth to postnatal day (P) 26. Animals were divided into three groups: Group A, Wistar rats as normal controls; Group B, HTX rats with a normal structure or only mild ventricular dilatation without any lesion in the white matter; and Group C, HTX rats with severe ventricular dilatation and cyst formation in the white matter. Group B rats showed similar development of GFAP-positive (GFAP+) cells to that in Group A rats, both morphologically and quantitatively. On the other hand, Group C rats showed definite structural changes in GFAP+ cells around the cystic cavities from P5. These included enriched cytoplasm and thickened cell processes with increased GFAP expression, and enveloped most cyst walls from P10. However, quantitative examination of the percentage of GFAP+ cells in Group C rats showed a similar developmental profile to those in Group A and B rats. Furthermore, the labeling index of BrdU-positive cells, indicating S-phase cells, in the white matter in Group C rats showed a similar decreasing pattern to that in Group A and B rats from P1 to P26.(ABSTRACT TRUNCATED AT 250 WORDS)

Related Papers

• → Glial fibrillary acidic protein - a cell type specific marker for Ito cells in vivo and in vitro(1996)175 cited
• → Specific monoclonal antibodies to glial fibrillary acidic protein (GFAP)(1986)15 cited
• → Glial fibrillary acidic protein in ependymomas and other brain tumors(1979)90 cited
• A glial fibrillary acidic protein(GFAP)positive cell line dervied from JCvirus induced medulloblastoma in hamster : JCウイルス誘発ハムスタ-髄芽腫由来のglial fibrillary acidic-protein(GFAP)陽性細胞株(2017)
• Time course degeneration and expression of glial fibrillary acidic protein in mer-knockout mice(2010)