Citations Over TimeTop 25% of 2007 papers
Abstract
Recent development in tissue engineering has enabled a new approach in tissue repair and reconstructive surgery using patient' s own cells. Human nasal septum cartilage is an optimum candidate as a starting material for this purpose. It is essential to understand the basic requirements for monolayer culture expansion of human nasal septum chondrocytes before using it in tissue engineering. The objective of this study was to investigate the effects of different media; Ham' s F12 Medium, Dulbecco' s Modified Eagle Medium (DMEM) or the equal combination of both media (F12:DMEM) on monolayer culture expansion of human nasal septum chondrocytes. A hydrogel, Pluronic F-127, was used as a biomaterial for cartilage reconstruction in nude mice with culture expanded chondrocytes. We demonstrated that F12:DMEM in 1:1 ratio promoted the highest human nasal septum chondrocytes proliferation and provided sufficient numbers of chondrocytes for cartilage engineering. This medium was effective for culture expansion to more than 2000 folds within 8 weeks. Although none of the medium maintained the chondrocytes' phenotype in long-term culture, the dedifferentiated cultured chondrocytes re-express collagen type II gene after being reconstructed into engineered tissue with Pluronic F-127. This hydrogel provided a 3-dimensional environment for cultured chondrocytes to regain their chondrocytic phenotype and thus initiate the generation of a good quality engineered cartilage.
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