Development of New PCR and Quantitative PCR Protocols for the Detection of <i>Plecoglossus altivelis</i> Poxvirus-like Virus in Atypical Cellular Gill Disease of Ayu
Fish Pathology2020Vol. 55(3), pp. 84–87
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T. Koyama, Daiki Komatsu, Tsubasa Uchino, Yuki Midorikawa, Goshi Kato, Takanori Ishikawa, Tomohiro Nishimura, Korenori Takeda, Hideo Fukuda, Shinpei Wada, Motohiko Sano
Abstract
Plecoglossus altivelis poxvirus-like virus (PaPV) is the presumptive causative agent of atypical cellular gill disease (ACGD), which has caused significant losses in aquaculture of ayu P. altivelis in Japan. In this study, we investigated a new PCR protocol and a quantitative PCR (qPCR) protocol with primers designed from the vaccinia virus A16L homologous gene sequence of PaPV. The new PCR showed a positive reaction for affected fish in all eight ACGD cases tested and no cross-reaction with carp edema virus, and was more sensitive than a PCR with BOKE30 primers. The qPCR quantified around 108–109 virus DNA copies/mg in the diseased fish gills.
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