Clastogenic and mito-inhibitory effect of heavy metals in root meristems of <i>Vicia faba</i>

Citations Over TimeTop 24% of 2015 papers

Abstract

Heavy metals are genotoxic and carcinogenic in nature. Chromosomal plant assay is an important tool used for the evaluation of genome damaging property of pollutants above the optimum level. The paper presents the results of a study concerning the influence of different concentrations of Pb and Cd on cytological and chromosomal level in root tip cells of Vicia faba L. The results revealed the specific variations in chromosomal behavior of root tip cells of Vicia faba L. depending on the nature of metals and their concentrations used for the seed treatment. The experimental study assesses the percentage of total active mitotic index and chromosomal abnormalities in the root meristems of Vicia faba L. at control and different doses of heavy metals. It was observed that a lower mitotic index and a higher frequency of chromosomal aberrations were established at all the doses of heavy metals as compared to control ones. An inverse relationship between the active mitotic index and doses of heavy metals was scored. The chromosomal abnormalities comprising C-mitosis, scattering, stickiness, precocious movement, laggard, bridges etc. were frequent along with the increasing doses of heavy metals. The treatment has been carried out at four different concentrations (2ppm, 4ppm, 6ppm, 8ppm) of both the metal salts (PbCO3 and CdCl2) at room temperature for 3 hrs. The results proved that Pb has more genotoxic potentialities than Cd.

Related Papers

• → Clastogenicity of the fungicide afugan in cultured human lymphocytes(2006)65 cited
• → Cytogenetic toxicity of methotrexate in mouse bone marrow(2000)49 cited
• → Genotoxic Effects of Herbicide Illoxan (Diclofop-Methyl) on Allium cepa L.(2009)22 cited
• → Clastogenic effect of pesticides in peripheral lymphocytes of cotton-field workers(1991)49 cited