Antibacterial and Biofilm Modulating Potential of Ferulic Acid-Grafted Chitosan against Human Pathogenic Bacteria
Citations Over TimeTop 11% of 2018 papers
Abstract
The emergence of more virulent forms of human pathogenic bacteria with multi-drug resistance is a serious global issue and requires alternative control strategies. The current study focused on investigating the antibacterial and antibiofilm potential of ferulic acid-grafted chitosan (CFA) against Listeria monocytogenes (LM), Pseudomonas aeruginosa (PA), and Staphylococcus aureus (SA). The result showed that CFA at 64 µg/mL concentration exhibits bactericidal action against LM and SA (>4 log reduction) and bacteriostatic action against PA (<2 log colony forming units/mL reduction) within 24 h of incubation. Further studies based on propidium iodide uptake assay, measurement of material released from the cell, and electron microscopic analysis revealed that the bactericidal action of CFA was due to altered membrane integrity and permeability. CFA dose dependently inhibited biofilm formation (52⁻89% range), metabolic activity (30.8⁻75.1% range) and eradicated mature biofilms, and reduced viability (71⁻82% range) of the test bacteria. Also, the swarming motility of LM was differentially affected at sub-minimum inhibitory concentration (MIC) concentrations of CFA. In the present study, the ability of CFA to kill and alter the virulence production in human pathogenic bacteria will offer insights into a new scope for the application of these biomaterials in healthcare to effectively treat bacterial infections.
Related Papers
- → The impact of quorum sensing and swarming motility on Pseudomonas aeruginosa biofilm formation is nutritionally conditional(2006)567 cited
- → Pseudomonas aeruginosa inhibits quorum-sensing mechanisms of soft rot pathogen Lelliottia amnigena RCE to regulate its virulence factors and biofilm formation(2022)30 cited
- → Synthesis and Biological Evaluation of Novel L-Homoserine Lactone Analogs as Quorum Sensing Inhibitors of <i>Pseudomonas aeruginosa</i>(2019)22 cited
- → Chemical Composition and Quorum Sensing Inhibitory Effect of Nepeta curviflora Methanolic Extract against ESBL Pseudomonas aeruginosa(2023)4 cited
- → Leaf Extracts of Selected Gardening Trees Can Attenuate Quorum Sensing and Pathogenicity of Pseudomonas aeruginosa PAO1(2017)5 cited