An efficient field and laboratory workflow for plant phylotranscriptomic projects
Applications in Plant Sciences2017Vol. 5(3)
Citations Over TimeTop 16% of 2017 papers
Ya Yang, Michael J. Moore, Samuel F. Brockington, Alfonso Timoneda, Tao Feng, Hannah E. Marx, Joseph F. Walker, Stephen A. Smith
Abstract
Our workflow is not only cost effective (ca. $270 per sample, as of August 2016, from tissue to reads) and time efficient (less than 50 h for 10-12 samples including all laboratory work and sample curation), but also has proven robust for extraction of difficult samples such as tissues containing high levels of secondary compounds.
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