Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency
Citations Over Time
Abstract
It is becoming increasingly apparent that electroporation is the most effective way to introduce plasmid DNA or siRNA into primary cells. The Gene Pulser MXcell electroporation system and Gene Pulser electroporation buffer (Bio-Rad) were specifically developed to easily transfect nucleic acids into mammalian cells and difficult-to-transfect cells, such as primary and stem cells. We will demonstrate how to perform a simple experiment to quickly identify the best electroporation conditions. We will demonstrate how to run several samples through a range of electroporation conditions so that an experiment can be conducted at the same time as optimization is performed. We will also show how optimal conditions identified using 96-well electroporation plates can be used with standard electroporation cuvettes, facilitating the switch from electroporation plates to electroporation cuvettes while maintaining the same electroporation efficiency. In the video, we will also discuss some of the key factors that can lead to the success or failure of electroporation experiments.
Related Papers
- → COMPARISON OF NON-VIRAL TRANSFECTION METHODS IN MELANOMA CELL PRIMARY CULTURES(2000)8 cited
- → Efficiency of DNA Transfection of Rat Heart Myoblast Cells H9c2(2-1) by Either Polyethyleneimine or Electroporation(2011)8 cited
- → A nonuniform electrical field electroporation chamber design(1989)2 cited
- Investigation on optimal parameters of electroporation-mediated transfection in 32DP210(2012)
- Comparison of electroporation and chemical agent transfection in gastric cancer cells with multidrug resistance(2015)