A Simple Way to Measure Ethanol Sensitivity in Flies
Journal of Visualized Experiments2011Iss. 48
Citations Over TimeTop 21% of 2011 papers
Abstract
Low doses of ethanol cause flies to become hyperactive, while high doses are sedating. The sensitivity to ethanol-induced sedation of a given fly strain is correlated with that strain s ethanol preference, and therefore sedation is a highly relevant measure to study the genetics of alcohol responses and drinking. We demonstrate a simple way to expose flies to ethanol and measure its intoxicating effects. The assay we describe can determine acute sensitivity, as well as ethanol tolerance induced by repeat exposure. It does not require a technically involved setup, and can therefore be applied in any laboratory with basic fly culture tools.
Related Papers
- → 4-Methylpyrazole, an alcohol dehydrogenase inhibitor, exacerbates alcohol-induced microencephaly during the brain growth spurt(1995)19 cited
- → Disorders of Excessive Alcohol Intake(1990)2 cited
- → ETHANOL CO-EXPOSURE INCREASES LETHALITY OF ALLYL ALCOHOL IN MALE SPRAGUE-DAWLEY RATS(1999)5 cited
- Glucose Consumption Assay for the Evaluation of Ethanol-producing Capability of Microzyme(2011)
- Impact of Caproate Acid,Wine Thick Tail to Low Degree of Ethyl Alcohol System(2014)