Operation of a Benchtop Bioreactor

Citations Over Time

Abstract

Fermentation systems are used to provide an optimal growth environment for many different types of cell cultures. The ability afforded by fermentors to carefully control temperature, pH, and dissolved oxygen concentrations in particular makes them essential to efficient large scale growth and expression of fermentation products. This video will briefly describe the advantages of the fermentor over the shake flask. It will also identify key components of a typical benchtop fermentation system and give basic instruction on setup of the vessel and calibration of its probes. The viewer will be familiarized with the sterilization process and shown how to inoculate the growth medium in the vessel with culture. Basic concepts of operation, sampling, and harvesting will also be demonstrated. Simple data analysis and system cleanup will also be discussed.

Related Papers

• → Dynamic Single-Use Bioreactors Used in Modern Liter- and m3- Scale Biotechnological Processes: Engineering Characteristics and Scaling Up(2013)58 cited
• → Bioreactors for Mammalian Cells(2014)33 cited
• → Novel Bioreactor Systems and Their Applications(1984)56 cited
• → Tubular bioreactors: Case study of bioreactor performance for industrial production and scientific research(1991)26 cited
• → Bioreactor for biopharmaceutical production: Simple controlled environment design(2018)1 cited