Insect Cell Extract Preparation by the Nitrogen Disruption Method for Cell-Free Translation
Abstract
Ainsect(exracS fr celfreeraRSawas prepared usaBob ce1 (srPiochambr a u(e devicehich(srs b()9icaaerdS by aerhe pressure ntheseerialS Thehespoenexract fbr cett-free transaton was prepared f the cell suspension at a deRsiy of 10108 cellsmi then disrupted at a nitregen pressure of 10 kgcma Furthermore synthesized proteins were stable in this extract but were degraded by proteaseahxtract prepared with a Potter-Elvenjem homogerrizer The insect cen extract showed efficient activity for translation of protein 2a axxd coat protein of the brome mosaic virus while inefficiently translating protein l a and protein 3a On the other hand mainly protein 3a and coat protein were translated in a wheat genm system while mainly proteins 2a and 3a were translated in a rabbit reticulocyte systern The insect cell-free system appears to possess an unusual property for enhancing translation of exogenous rnRNA making it differeRt from the rabbit riculocyte and wheat gesystems have steadily increased (MadiR et al 2000 Kawarasaki et ag 1995 Nakano et ag 1994 K 1996)1ghiss(ttcowihvophe Laboratory of Microbial Geneties Technelogy
Related Papers
- → Insect Galls(1982)56 cited
- → Chiral chemodiversity and its role for biological activity. Some observations from studies on insect/insect and insect/plant relationships(1996)33 cited
- → A method for predicting nitrogen gas pores in nitrogen alloying stainless steels(2005)14 cited
- Effects of Nitrogen Application Period on Nitrogen Accumulation,Translocation and Nitrogen Use Efficiency of Summer Maize(2011)
- Research on Nitrogen-controlling Process for Melting Nitrogen-contained 316L Stainless Steel in VIM Furnace(2013)