Elongated Nanoparticle Aggregates in Cancer Cells for Mechanical Destruction with Low Frequency Rotating Magnetic Field
Citations Over TimeTop 10% of 2017 papers
Abstract
Magnetic nanoparticles (MNPs) functionalized with targeting moieties can recognize specific cell components and induce mechanical actuation under magnetic field. Their size is adequate for reaching tumors and targeting cancer cells. However, due to the nanometric size, the force generated by MNPs is smaller than the force required for largely disrupting key components of cells. Here, we show the magnetic assembly process of the nanoparticles inside the cells, to form elongated aggregates with the size required to produce elevated mechanical forces. We synthesized iron oxide nanoparticles doped with zinc, to obtain high magnetization, and functionalized with the epidermal growth factor (EGF) peptide for targeting cancer cells. Under a low frequency rotating magnetic field at 15 Hz and 40 mT, the internalized EGF-MNPs formed elongated aggregates and generated hundreds of pN to dramatically damage the plasma and lysosomal membranes. The physical disruption, including leakage of lysosomal hydrolases into the cytosol, led to programmed cell death and necrosis. Our work provides a novel strategy of designing magnetic nanomedicines for mechanical destruction of cancer cells.
Related Papers
- → Iron oxide nanoparticles for neuronal cell applications: uptake study and magnetic manipulations(2016)169 cited
- → Magnetic nanoparticles as potential candidates for biomedical and biological applications(2015)46 cited
- → Magnetic Patterning of Tissue Spheroids Using Polymer Microcapsules Containing Iron Oxide Nanoparticles(2021)18 cited
- → Magnetic force microscopy of iron oxide nanoparticles and their cellular uptake(2009)28 cited
- → Dense covalent attachment of magnetic iron oxide nanoparticles onto silica particles using a diazonium salt chemistry approach(2012)14 cited