Comparative proteomics of Rhizopus delemar ATCC 20344 unravels the role of amino acid catabolism in fumarate accumulation
PeerJ2017Vol. 5, pp. e3133–e3133
Citations Over TimeTop 21% of 2017 papers
Dorett I. Odoni, Juan Antonio Tamayo‐Ramos, Jasper Sloothaak, Ruben Heck, Vítor A. P. Martins dos Santos, Leo H. de Graaff, María Suárez‐Diez, Peter J. Schaap
Abstract
The filamentous fungus Rhizopus delemar naturally accumulates relatively high amounts of fumarate. Although the culture conditions that increase fumarate yields are well established, the network underlying the accumulation of fumarate is not yet fully understood. We set out to increase the knowledge about fumarate accumulation in R. delemar. To this end, we combined a transcriptomics and proteomics approach to identify key metabolic pathways involved in fumarate production in R. delemar, and propose that a substantial part of the fumarate accumulated in R. delemar during nitrogen starvation results from the urea cycle due to amino acid catabolism.
Related Papers
- → Metabolic Fate of Fumarate, a Side Product of the Purine Salvage Pathway in the Intraerythrocytic Stages of Plasmodium falciparum(2011)82 cited
- → The Citric Acid Cycle(1998)4 cited
- → Tricarboxylic acid cycle enzymes of a pseudophyllid cestode Penetrocephalus ganapatii(1990)1 cited
- → CATABOLISM OF SIMPLE UNITS(1981)
- → Faculty Opinions recommendation of Fumarate reductase activity maintains an energized membrane in anaerobic Mycobacterium tuberculosis.(2011)