Whole‐Cell Patch‐Clamp Electrophysiology of Voltage‐Sensitive Channels
Current Protocols in Toxicology2003Vol. 17(1), pp. Unit11.12–Unit11.12
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Abstract
Ion channels are critical modulators of electrical excitability in neurons and are targets of a wide variety of neurotoxicants, including metals and insecticides. The only way to directly examine effects of toxicants on ion channel function in an individual cell on a physiologically relevant time scale is to utilize patch-clamp electrophysiological techniques. This unit presents the basics of utilizing whole-cell patch-clamp techniques to study effects of toxicants on voltage-sensitive Ca(2+) channels in neurons grown in culture.
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