Continuous dehydrogenation of a steroid with immobilized microbial cells: Effect of an exogenous electron acceptor
Citations Over TimeTop 12% of 1978 papers
Abstract
Abstract Whole cells of Pseudomonas testosteroni , induced to synthesize steroid‐transforming enzymes beforehand, have been immobilized by entrapment in polyacrylamide gel. The immobilized cells have then been used to catalyze the continuous Δ 1 ‐dehydrogenation of Reichstein's substance S under various conditions in the presence of phenazine methosulfate (PMS), an electron acceptor for the cell‐free Δ 1 ‐dehydrogenase. The presence of PMS substantially increases the rate of reaction when fed with the steroid substrate to a continuous stirred tank reactor containing the immobilized cells. The operational half‐life of the Δ 1 ‐dehydrogenase activity of the cells, about 103 hr under the best operating conditions, is essentially unaffected by the presence of PMS. Though the acceleration of the reaction may be due to PMS‐mediated passage of electrons from some component in the electron transport chain to molecular oxygen, the lack of a similar effect with methylene blue is consistent with the conclusion that PMS functions directly as the electron acceptor for the Δ 1 ‐dehydrogenase.
Related Papers
- → Bis(phenylsulfone) as a strong electron acceptor of thermally activated delayed fluorescent emitters(2016)8 cited
- → Spectroscopic and Electrical Properties of Polymeric Donor—Acceptor Complexes(1967)14 cited
- → Light Induced Electron Transfer between Spatially Separated Donor and Acceptor(1978)
- → ChemInform Abstract: ELECTRON DONOR‐ACCEPTOR COMPOUNDS. XXIX. ELECTRON DONOR‐ACCEPTOR PARACYCLOPHANES WITH 7,7,8,8‐TETRACYANOQUINODIMETHANE (TCNQ) AS ACCEPTOR UNIT(1983)
- → Dehydrogenation – Biological(2002)