A cytochemical assay for osteoclast cathepsin K activity
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Abstract
Abstract Cathepsin K is a member of the papain superfamily of cysteine proteases and plays a pivotal role in osteoclast‐mediated bone resorption. This enzyme is an excellent target for antiresorptive therapies for osteopenic disorders such as osteoporosis. 1 Although isolated inhibitor studies on purified enzymes is required to discover potent and selective inhibitors of cathepsin K, a quantitative cytochemical assay 2 for cathepsin K would allow inhibitors to be tested on actual osteoclasts within sections of bone. Furthermore cathepsin K activity could be used to identify and analyse osteoclasts at definitive stages of their lifespan. A cytochemical assay is described that localizes osteoclast cathepsin K activity in unfixed, undecalcified cryostat sections of animal and human bone. Copyright © 2003 John Wiley & Sons, Ltd.
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