Using Evans Blue Dye to Determine Blood‐Brain Barrier Integrity in Rodents
Current Protocols in Immunology2019Vol. 126(1), pp. e83–e83
Citations Over TimeTop 10% of 2019 papers
Abstract
The blood-brain barrier (BBB) is an active and selective barrier that shields the brain from endogenous and exogenous insults. Different stimuli may lead to the disruption of this barrier, including inflammation and trauma. Several methods are used to evaluate BBB disruption. The most widely used method is Evans blue (EB) dye extravasation. EB cannot normally pass through the BBB and thus its presence in brain tissue indicates alterations in permeability. This protocol details the steps of EB extravasation in rodents. Important aspects regarding critical steps and advantages are also provided. © 2019 by John Wiley & Sons, Inc.
Related Papers
- → Influence of Nanoparticles on Blood–Brain Barrier Permeability and Brain Edema Formation in Rats(2009)206 cited
- → Using Evans Blue Dye to Determine Blood‐Brain Barrier Integrity in Rodents(2019)164 cited
- → Measurement of Blood-Brain Barrier Hyperpermeability Using Evans Blue Extravasation Assay(2023)11 cited
- → Blood-cerebrospinal fluid barrier in schizophrenic patients(1988)2 cited
- → Assay of Blood Brain Barrier and Placental Barrier Permeability(2013)