An efficient solid‐phase synthesis of peptidyl‐N‐acetylguanidines for use in native chemical ligation
Citations Over TimeTop 25% of 2016 papers
Abstract
In the modern protocols of chemical protein syntheses, peptide-α-thioesters have been used as key components for the assembly of full-length polypeptides through chemoselective peptide coupling reactions. A variety of thioester precursors have been developed for the synthesis of the peptide-α-thioesters by Fmoc solid phase peptide synthesis (Fmoc-SPPS). Recently our group found a peptidyl-N-acetylguanidine as a new peptide-α-thioester precursor. This peptide derivative can be converted into a corresponding peptide-α-thioester only by treatment with an excess amount of a thiol in aqueous buffers at around neutral pH. This unique property allowed us to envision the practical use of the peptidyl-N-acetylguanidines for the chemical syntheses of proteins; however, an efficient synthetic method has been lacking. Herein, we report an efficient solid-phase synthesis of peptidyl-N-acetylguanidines. This new synthetic method employing selective activation and cleavage of a peptide bond successfully provided peptidyl-N-acetylguanidines from the on-resin protected peptides prepared by standard Fmoc-SPPS. We also evaluated the reactivity of a peptidyl-N-acetylguanidine in native chemical ligation through the synthesis of glucose-dependent insulinotropic polypeptide analogue. Copyright © 2016 European Peptide Society and John Wiley & Sons, Ltd.
Related Papers
- → Extending the Applicability of Native Chemical Ligation(1996)267 cited
- → Peptide thioester preparation by Fmoc solid phase peptide synthesis for use in native chemical ligation(2000)106 cited
- → Solid phase synthesis of peptide-selenoesters(2013)33 cited
- → An efficient solid‐phase synthesis of peptidyl‐N‐acetylguanidines for use in native chemical ligation(2016)8 cited
- → Peptide thioester preparation by Fmoc solid phase peptide synthesis for use in native chemical ligation(2000)3 cited