Determination of protein phosphorylation sites by mass spectrometry: a novel electrospray‐based method
Rapid Communications in Mass Spectrometry2005Vol. 19(22), pp. 3343–3348
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Vera Alverdi, Francesca Di Pancrazio, Giovanna Lippe, Carlo Pucillo, Bruno Casetta, Gennaro Esposito
Abstract
Several methods are used to identify protein phosphorylation sites. We report a novel electrospray-based method for the determination of phosphorylation sites by mass spectrometry, using two different declustering potential values. This method allows one to obtain, with a single liquid chromatography/mass spectrometry (LC/MS) run, the pattern with either the phosphorylated or the unphosphorylated species of a protein tryptic digest, that can be further analyzed by tracing back the origin of each HPO3-deprived form using the capabilities of tandem mass spectrometers.
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