Evaluation and minimization of nonspecific tryptic cleavages in proteomic sample preparation
Rapid Communications in Mass Spectrometry2020Vol. 34(10), pp. e8733–e8733
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Abstract
The significant decrease in nonspecific cleavages and artificial modifications improves the accuracy of protein quantification and the identification of low-abundance proteins, and it is especially useful for studying protein posttranslational modifications. For trypsin digestion, the proposed 2-h protocol can potentially be a replacement for the traditional 16-h protocol.
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