Purification and properties of the lipase fromCandida deformans (zach) langeron and guerra

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Abstract

Abstract Palm oil being solid at room temperature could be converted into a fluid oil by substitution of about 40–50% of its palmitic acid. This could be achieved by a fermentation process or using yeast lipase. Candida deformans CBS 2071 seemed suitable for this purpose: therefore, its lipase was isolated and studied. This enzyme was purified by acetone precipitation followed by chromatographies on Sephadex C 50 and Sephadex G 150. The purification factor achieved was ×70, and the protein and activity yields were 0.25% and 18%, respectively. The homogeneity of the purified enzyme was verified by polyacrylamide gel electrophoresis. The enzyme molecular weight was estimated at 207,000. Its activity is optimal between 40 C and 50 C and its optimum pH is 7.0. This enzyme is thermo‐resistant and loses only 14% and 18% of its activity, respectively, when heated to 45 C and 55 C for 30 min. Its activation energy was 2.75 kcal.mole −1 and its inactivation energy was around 21 kcal.mole −1 . This enzyme is activated by Ca ++ , Mg ++ and Co ++ and inhibited by Cu ++ , Zn ++ , and p‐chloromercuribenzoate (pCMB) and EDTA. The synthesis of this lipase is induced by lipid substrates in the culture medium and inhibited by glucose. This enzyme attacks primarily the 1‐ (or 3‐) position of all triglycerides tested. Hydrolysis was preferential for triglycerides containing short chain fatty acids. The triglycerides with monounsaturated monoacids were more quickly hydrolyzed, than those with saturated monoacids. The presence of two and especially three double bonds in the fatty acid chain seemed to slow down the rate of hydrolysis.

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