The Hydrophobins HFBI and HFBII from Trichoderma reesei Showing Efficient Interactions with Nonionic Surfactants in Aqueous Two-Phase Systems

Citations Over TimeTop 15% of 2001 papers

Abstract

Fungal hydrophobins are a group of surface active, self-assembling proteins. The filamentous fungus Trichoderma reesei produces two (class II) hydrophobins, HFBI and HFBII. We have studied how these water-soluble hydrophobins behave in two-phase systems using a series of nonionic surfactants with different characteristics. It was found that both hydrophobins, but especially HFBI, had a very high affinity for the surfactants. The highest partitioning coefficient, over 2500, was observed for HFBI with C(11)EO(2). Reducing the disulfides in the protein resulted in a complete loss of affinity for the surfactant, which demonstrates that the interaction is dependent on the disulfide-stabilized conformation. The hydrophobins could be efficiently extracted back from the surfactant phase by addition of alcohols such as isobutanol. Effects of the type of surfactant, temperature, pH, and ionic strength were investigated. The use of this method for purifying the proteins from crude fungal culture supernatants is demonstrated and implications of the protein-polymer interaction are discussed.

Related Papers

• → Transition between a Pickering Emulsion and an Oil-in-Dispersion Emulsion Costabilized by Alumina Nanoparticles and a Cationic Surfactant(2020)51 cited
• → Formation of a liquid crystalline phase between aqueous surfactant solutions and oily substances(1990)6 cited
• Studies of the adsorption of polyglycerol polyricinileates at the paraffin oil/aqueous acetic acid solution interface and the effect of PGPR on the phase behavior of the system(2012)
• Progress and applications of aqueous surfactant two-phase system(2008)
• Property of Aqueous Two-Phase System Containing Alkyltrimethylammonium Bromides and Sodium Dodecyl Sulfate(2009)