Methyl Groups as Probes for Proteins and Complexes in In-Cell NMR Experiments
Journal of the American Chemical Society2004Vol. 126(22), pp. 7119–7125
Citations Over TimeTop 10% of 2004 papers
Zach Serber, Wesley Straub, Lorenzo Corsini, Anson M. Nomura, Nobuhisa Shimba, Charles S. Craik, Paul Ortiz de Montellano, Volker Dötsch
Abstract
Studying protein components of large intracellular complexes by in-cell NMR has so far been impossible because the backbone resonances are unobservable due to their slow tumbling rates. We describe a methodology that overcomes this difficulty through selective labeling of methyl groups, which possess more favorable relaxation behavior. Comparison of different in-cell labeling schemes with three different proteins, calmodulin, NmerA, and FKBP, shows that selective labeling with [(13)C]methyl groups on methionine and alanine provides excellent sensitivity with low background levels at very low costs.
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