Tet-Mediated Formation of 5-Hydroxymethylcytosine in RNA
Journal of the American Chemical Society2014Vol. 136(33), pp. 11582–11585
Citations Over TimeTop 10% of 2014 papers
Lijuan Fu, Candace Guerrero, Na Zhong, Nicholas J. Amato, Yunhua Liu, Shuo Liu, Qian Cai, Debin Ji, Seung‐Gi Jin, Laura J. Niedernhofer, Gerd P. Pfeifer, Guo-Liang Xu, Yinsheng Wang
Abstract
Oxidation of 5-methylcytosine in DNA by ten-eleven translocation (Tet) family of enzymes has been demonstrated to play a significant role in epigenetic regulation in mammals. We found that Tet enzymes also possess the activity of catalyzing the formation of 5-hydroxymethylcytidine (5-hmrC) in RNA in vitro. In addition, the catalytic domains of all three Tet enzymes as well as full-length Tet3 could induce the formation of 5-hmrC in human cells. Moreover, 5-hmrC was present at appreciable levels (∼1 per 5000 5-methylcytidine) in RNA of mammalian cells and tissues. Our results suggest the involvement of this oxidation in RNA biology.
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