A DNA aptamer recognising a malaria protein biomarker can function as part of a DNA origami assembly
Citations Over TimeTop 10% of 2016 papers
Abstract
Abstract DNA aptamers have potential for disease diagnosis and as therapeutics, particularly when interfaced with programmable molecular technology. Here we have combined DNA aptamers specific for the malaria biomarker Plasmodium falciparum lactate dehydrogenase ( Pf LDH) with a DNA origami scaffold. Twelve aptamers that recognise Pf LDH were integrated into a rectangular DNA origami and atomic force microscopy demonstrated that the incorporated aptamers preserve their ability to specifically bind target protein. Captured Pf LDH retained enzymatic activity and protein-aptamer binding was observed dynamically using high-speed AFM. This work demonstrates the ability of DNA aptamers to recognise a malaria biomarker whilst being integrated within a supramolecular DNA scaffold, opening new possibilities for malaria diagnostic approaches based on DNA nanotechnology.
Related Papers
- → Aptamer cocktails: Enhancement of sensing signals compared to single use of aptamers for detection of bacteria(2013)87 cited
- → An aptamer cocktail-based electrochemical aptasensor for direct capture and rapid detection of tetracycline in honey(2019)40 cited
- → Idiosyncrasies of Thermofluorimetric Aptamer Binding Assays(2019)10 cited
- → Selecting DNA aptamers for endotoxin separation(2015)5 cited
- → Aptameric sensors based on structural change for diagnosis(2010)9 cited