Deletion of RD1 fromMycobacterium tuberculosisMimics Bacille Calmette‐Guérin Attenuation
Citations Over TimeTop 1% of 2002 papers
Abstract
The tuberculosis (TB) vaccine bacille Calmette-Guérin (BCG) is a live attenuated organism, but the mutation responsible for its attenuation has never been defined. Recent genetic studies identified a single DNA region of difference, RD1, which is absent in all BCG strains and present in all Mycobacterium tuberculosis (MTB) strains. The 9 open-reading frames predicted within this 9.5-kb region are of unknown function, although they include the TB-specific immunodominant antigens ESAT-6 and CFP-10. In this study, RD1 was deleted from MTB strain H37Rv, and virulence of H37Rv:DeltaRD1 was assessed after infections of the human macrophage-like cell line THP-1, human peripheral blood monocyte-derived macrophages, and C57BL/6 mice. In each of these systems, the H37Rv:DeltaRD1 strain was strikingly less virulent than MTB and was very similar to BCG controls. Therefore, it was concluded that genes within or controlled by RD1 are essential for MTB virulence and that loss of RD1 was important in BCG attenuation.
Related Papers
- → Transcriptional signatures of Mycobacterium tuberculosis in mouse model of intraocular tuberculosis(2019)9 cited
- → Transcriptional Biomarkers of Differentially Detectable Mycobacterium tuberculosis in Patient Sputum(2022)9 cited
- [Virulence of Mycobacterium tuberculosis. II. Comparison of virulence of Mycobacterium tuberculosis constantly exposed to oxygen or nitrogen flow based upon a recently introduced virulence test pathogenicity standard].(1959)
- → ESAT-6 Independent Survival and Virulence:Need For Paradigm Shift?(2018)
- → Research progress on virulence-related genes of Mycobacterium tuberculosis(2010)