PSL-LCCL: a resource for subcellular protein localization in liver cancer cell line SK_HEP1
Citations Over Time
Abstract
The characterization of subcellular protein localization provides a basis for further understanding cellular behaviors. A delineation of subcellular localization of proteins on cytosolic membrane-bound organelles in human liver cancer cell lines (hLCCLs) has yet to be performed. To obtain its proteome-wide view, we isolated and enriched six cytosolic membrane-bound organelles in one of the hLCCLs (SK_HEP1) and quantified their proteins using mass spectrometry. The vigorous selection of marker proteins and a machine-learning-based algorithm were implemented to localize proteins at cluster and neighborhood levels. We validated the performance of the proposed method by comparing the predicted subcellular protein localization with publicly available resources. The profiles enabled investigating the correlation of protein domains with their subcellular localization and colocalization of protein complex members. A subcellular proteome database for SK_HEP1, including (i) the subcellular protein localization and (ii) the subcellular locations of protein complex members and their interactions, was constructed. Our research provides resources for further research on hLCCLs proteomics. Database URL: http://www.igenetics.org.cn/project/PSL-LCCL/.
Related Papers
- → A review from biological mapping to computation-based subcellular localization(2023)19 cited
- → PSL-LCCL: a resource for subcellular protein localization in liver cancer cell line SK_HEP1(2021)5 cited
- Advancement of Protein Subcellular Localization in Plants(2006)
- → Systematic Subcellular Localization of Novel Proteins(2006)
- → Investigating 14-3-3 Protein Subcellular Localization, Colocalization with Subcellular Markers and Interaction with Rac1(2019)