Pax7-expressing satellite cells are indispensable for adult skeletal muscle regeneration
Citations Over TimeTop 1% of 2011 papers
Abstract
Distinct cell populations with regenerative capacity have been reported to contribute to myofibres after skeletal muscle injury, including non-satellite cells as well as myogenic satellite cells. However, the relative contribution of these distinct cell types to skeletal muscle repair and homeostasis and the identity of adult muscle stem cells remain unknown. We generated a model for the conditional depletion of satellite cells by expressing a human diphtheria toxin receptor under control of the murine Pax7 locus. Intramuscular injection of diphtheria toxin during muscle homeostasis, or combined with muscle injury caused by myotoxins or exercise, led to a marked loss of muscle tissue and failure to regenerate skeletal muscle. Moreover, the muscle tissue became infiltrated by inflammatory cells and adipocytes. This localised loss of satellite cells was not compensated for endogenously by other cell types, but muscle regeneration was rescued after transplantation of adult Pax7(+) satellite cells alone. These findings indicate that other cell types with regenerative potential depend on the presence of the satellite cell population, and these observations have important implications for myopathic conditions and stem cell-based therapeutic approaches.
Related Papers
- → Principles of Regenerative Biology(2007)245 cited
- → Importance of Regional Regeneration Capacity in General Type of Neighborhood Regeneration in Urban Regeneration Projects - The Cases of Changsin/Sungin-Dong, Haebangchon, and Garibong-Dong in Seoul -(2019)3 cited
- → Some Problems of Regeneration(1961)14 cited
- → Resources of regeneration in planarians(2006)2 cited
- Newt Lens Regeneration: Role of Oct-4 in Newt Regenerating Tissue and Proteome Analysis of Regeneration Competent Vs. Regeneration Incompetent Cells(2014)