Stage-specific inductive signals in theDrosophilaneuroectoderm control the temporal sequence of neuroblast specification
Citations Over Time
Abstract
One of the initial steps of neurogenesis in the Drosophila embryo is the delamination of a stereotype set of neural progenitor cells (neuroblasts) from the neuroectoderm. The time window of neuroblast segregation has been divided into five successive waves (S1-S5) in which subsets of neuroblasts with specific identities are formed. To test when identity specification of the various neuroblasts takes place and whether extrinsic signals are involved, we have performed heterochronic transplantation experiments. Single neuroectodermal cells from stage 10 donor embryos (after S2) were transplanted into the neuroectoderm of host embryos at stage 7 (before S1) and vice versa. The fate of these cells was uncovered by their lineages at stage 16/17. Transplanted cells adjusted their fate to the new temporal situation. Late neuroectodermal cells were able to take over the fate of early (S1/S2) neuroblasts. The early neuroectodermal cells preferentially generated late (S4/S5) neuroblasts, despite their reduced time of exposure to the neuroectoderm. Furthermore, neuroblast fates are independent from divisions of neuroectodermal progenitor cells. We conclude from these experiments that neuroblast specification occurs sequentially under the control of non-cell-autonomous and stage-specific inductive signals that act in the neuroectoderm.
Related Papers
- → The pattern of neuroblast formation, mitotic domains and proneural gene expression during early brain development in Drosophila(2003)123 cited
- → Stage-specific inductive signals in theDrosophilaneuroectoderm control the temporal sequence of neuroblast specification(2001)21 cited
- 초파리 중추신경 개체성 확립과 형성에 미치는 중앙선 세포의 기능(1999)
- → Faculty Opinions recommendation of The pattern of neuroblast formation, mitotic domains and proneural gene expression during early brain development in Drosophila.(2003)
- → Adult neurogenesis in the hippocampus(2007)