Novel fixed Z-dimension (FiZD) kidney primordia and an organoid culture system for time-lapse confocal imaging
Citations Over TimeTop 19% of 2017 papers
Abstract
Tissue, organ and organoid cultures provide suitable models for developmental studies, but our understanding of how the organs are assembled at the single-cell level still remains unclear. We describe here a novel fixed z-direction (FiZD) culture setup that permits high-resolution confocal imaging of organoids and embryonic tissues. In a FiZD culture a permeable membrane compresses the tissues onto a glass coverslip and the spacers adjust the thickness, enabling the tissue to grow for up to 12 days. Thus, the kidney rudiment and the organoids can adjust to the limited z-directional space and yet advance the process of kidney morphogenesis, enabling long-term time-lapse and high-resolution confocal imaging. As the data quality achieved was sufficient for computer-assisted cell segmentation and analysis, the method can be used for studying morphogenesis ex vivo at the level of the single constituent cells of a complex mammalian organogenesis model system.
Related Papers
- → Photoinduction of Flower Identity in Vegetatively Biased Primordia(1998)52 cited
- → DEVELOPMENTAL POTENTIALITIES OF LEAF PRIMORDIA OF OSMUNDA CINNAMOMEA: THE INFLUENCE OF DETERMINED LEAF PRIMORDIA ON UNDETERMINED LEAF PRIMORDIA(1967)14 cited
- → Developmental potentialities of leaf primordia of Osmunda cinnamomea. II. Further studies on the influence of determined leaf primordia on undetermined leaf primordia(1969)8 cited
- Studies on Dynamic Models and Characteristics of Apex Primordium Differentiation in Wheat(2000)